NMR structural studies of membrane proteins

Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins.

A large portion of proteins in living organisms are membrane proteins which play critical roles in the biology of the cell, from maintenance of the biological membrane integrity to communication of cells with their surroundings. To understand their mechanism of action, structural information is essential. Nevertheless, structure determination of transmembrane proteins is still a challenging are...

Structural studies of proteins by paramagnetic solid-state NMR spectroscopy.

Paramagnetism-based nuclear pseudocontact shifts and spin relaxation enhancements contain a wealth of information in solid-state NMR spectra about electron-nucleus distances on the ∼20 Å length scale, far beyond that normally probed through measurements of nuclear dipolar couplings. Such data are especially vital in the context of structural studies of proteins and other biological molecules th...

Structural studies of membrane proteins using transmission electron microscopy

Overexpression of membrane proteins in mammalian cells for structural studies

The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total number of membrane proteins encoded by a mammalian genome. In addition, the str...

Functional and Structural Studies on Heptahelical Membrane Proteins

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